CCL17 ELISA Kit

Catalog No. ABIN6720150

Supplier Product No.: koa0394

This Human CCL17 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CCL17.

Quick Overview for CCL17 ELISA Kit (ABIN6720150)

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CCL17 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0394

Supplier: Rockland

Purpose: Human CCL17 - TARC Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CCL17. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 31.2 pg/mL-2000 pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CCL17 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL17 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: A24-S94

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CCL17

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Alternative Name: CCL17/TARC

Background:

Synonyms: A 152E5.3, ABCD 2, C C motif chemokine 17, C-C motif chemokine 17, CC chemokine TARC, CCL 17, CCL17, CCL17_HUMAN, Chemokine (C C motif) ligand 17, Chemokine ligand 17, MGC138271, MGC138273, SCYA17, Small inducible cytokine A17, Small inducible cytokine subfamily A (Cys Cys) member 17, Small inducible cytokine subfamily A member 17, Small-inducible cytokine A17, T cell directed CC chemokine, Thymus and activation regulated chemokine, Thymus and activation-regulated chemokine

Background: Chemokine(C-C motif) ligand 17(CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine(TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.

Gene ID: 6361

NCBI Accession: NP_002978

UniProt: Q92583