CSF3R ELISA Kit

Catalog No. ABIN6720159

Supplier Product No.: koa0452

This Human CSF3R ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CSF3R.

Quick Overview for CSF3R ELISA Kit (ABIN6720159)

Target: CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CSF3R ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0452

Supplier: Rockland

Purpose: Human CSF3R - G-CSF R Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CSF3R. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CSF3R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CSF3R standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.cNSO, Immunogen sequence: E25-P621

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CSF3R

Target: CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))

Alternative Name: CSF3R/G-CSF R

Background:

Synonyms: CD 114, CD114, CD114 antigen, Colony stimulating factor 3 receptor (granulocyte), Colony stimulating factor 3 receptor, CSF 3R, CSF3R, CSF3R_HUMAN, Csfgr, G CSF R, G-CSF receptor, G-CSF-R, GCSFR, Granulocyte colony stimulating factor receptor, Granulocyte colony-stimulating factor receptor, OTTHUMP00000009703, OTTHUMP00000009704, OTTHUMP00000009705

Background: The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2.

Gene ID: 1441

NCBI Accession: NP_000751

UniProt: Q99062