CXCL9 ELISA Kit

Catalog No. ABIN6720161

Supplier Product No.: koa0427

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CXCL9.

Quick Overview for CXCL9 ELISA Kit (ABIN6720161)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CXCL9 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0427

Supplier: Rockland

Purpose: Human CXCL9 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CXCL9. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CXCL9 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: T23-T125

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Alternative Name: CXCL9

Background:

Synonyms: C-X-C motif chemokine 9, Chemokine (C-X-C motif) ligand 9, CMK, Crg 10, CXCL9, CXCL9_HUMAN, Gamma interferon induced monokine, Gamma-interferon-induced monokine, HuMIG, MIG, Monokine induced by gamma interferon, Monokine induced by interferon gamma, Monokine induced by interferon-gamma, SCYB9, Small inducible cytokine B9, Small inducible cytokine subfamily B member 9, Small-inducible cytokine B9

Background: Chemokine(C-X-C motif) ligand 9(CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon(MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4.1, 2 CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3.3 The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

Gene ID: 4283

NCBI Accession: NP_002407

UniProt: Q07325