IL18R1 ELISA Kit

Catalog No. ABIN6720173

Supplier Product No.: koa0744

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL18R1.

Quick Overview for IL18R1 ELISA Kit (ABIN6720173)

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details IL18R1 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0744

Supplier: Rockland

Purpose: Human IL-18R1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human IL-18R1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-18R1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-18R1 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: C22-R329

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IL18R1

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Alternative Name: IL-18R1

Background:

Synonyms: CD218 antigen-like family member A, CD218a, CDw218a, CDw218a antigen, IL 1Rrp, IL-18R-1, IL-18R1, IL-1Rrp, IL1 receptor related protein, IL1 receptor-related protein, IL18R_HUMAN, IL18R1, IL18RA, Il18ralpha, IL1R-rp, IL1RRP, Interleukin 18 receptor 1, Interleukin 18 receptor alpha chain, Interleukin-18 receptor 1

Background: The interleukin-18 receptor 1(IL-18R1), also known as CDw218a(cluster of differentiation w218a) or IL18RA, is an interleukin receptor of the immunoglobulin superfamily. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2(IL-1Rrp2), and IL1RL1(T1/ST2), form a gene cluster on chromosome 2q. This receptor specifically binds interleukin 18(IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells.

Gene ID: 8809

NCBI Accession: NP_001269328

UniProt: Q13478