CD137 ELISA Kit

Catalog No. ABIN6720180

Supplier Product No.: koa0616

The Human CD137 ELISA Kit (ABIN6720180) is a Colorimetric ELISA Kit designed to quantify Human CD137.

Quick Overview for CD137 ELISA Kit (ABIN6720180)

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CD137 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0616

Supplier: Rockland

Purpose: Human TNFRSF9 - 4-1BB Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human TNFRSF9. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TNFRSF9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TNFRSF9 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: L24-Q186

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CD137

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Alternative Name: TNFRSF9/4-1BB

Background:

Synonyms: 4-1BB, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, Homolog of mouse 4 1BB, ILA, Induced by lymphocyte activation (ILA), Interleukin activated receptor homolog of mouse Ly63, Receptor protein 4 1BB, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, TNR9_HUMAN, Tumor necrosis factor receptor superfamily member 9

Background: CD137 is a member of the tumor necrosis factor(TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9(TNFRSF9), 4-1BB and induced by lymphocyte activation(ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Gene ID: 3604

NCBI Accession: NP_001552

UniProt: Q07011

Pathways: Cancer Immune Checkpoints