Thymic Stromal Lymphopoietin ELISA Kit

Catalog No. ABIN6720181

Supplier Product No.: koa0588

Human Thymic Stromal Lymphopoietin ELISA Kit Colorimetric assay for quantification of Human Thymic Stromal Lymphopoietin.

Quick Overview for Thymic Stromal Lymphopoietin ELISA Kit (ABIN6720181)

Target: Thymic Stromal Lymphopoietin (TSLP)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details Thymic Stromal Lymphopoietin ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0588

Supplier: Rockland

Purpose: Human TSLP Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human TSLP. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TSLP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human TSLP standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: Y29-Q159

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background:

Synonyms: Thymic stromal lymphopoietin, Thymic stromal lymphopoietin protein TSLP, Tslp, TSLP protein, TSLP_HUMAN

Background: Thymic stromal lymphopoietin, also called TSLP is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2(TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases.

Gene ID: 85480

NCBI Accession: NP_149024

UniProt: Q969D9