CD32-B/C ELISA Kit

Catalog No. ABIN6720188

Supplier Product No.: koa0786

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse CD32-B/C.

Quick Overview for CD32-B/C ELISA Kit (ABIN6720188)

Target: CD32-B/C

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0786

Supplier: Rockland

Purpose: Mouse CD32 - FCGR2b - c Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse CD32. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CD32 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD32 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: T40-R217

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: CD32-B/C

Alternative Name: CD32/FCGR2b/c

Background:

Synonyms: CD32 antigen, CD32A, CD32B, CD32C, CDw32, Fc fragment of IgG low affinity IIa receptor, Fc fragment of IgG low affinity IIb receptor, Fc fragment of IgG low affinity IIc receptor, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, FC-gamma-RIIB, FCG2, FCG2A_HUMAN, FcgammaRIIC, FcGR, FCGR2, FCGR2A, FCGR2C, Fcgr3, Fcgr3a, Fcr-2, Fcr-3, FcRII a, FCRII, FcRII b, FcRIIC, IGFR 2, IGFR2, IgG Fc receptor II a, IgG Fc receptor II a, Low affinity immunoglobulin gamma Fc region receptor II, Lymphocyte antigen 17

Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Gene ID: 14130

NCBI Accession: NP_034317

UniProt: P08101