Cathepsin L ELISA Kit

Catalog No. ABIN6720215

Supplier Product No.: koa0389

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Cathepsin L.

Quick Overview for Cathepsin L ELISA Kit (ABIN6720215)

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details Cathepsin L ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Supplier Product No.: koa0389

Supplier: Rockland

Purpose: Human Cathepsin L Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human Cathepsin L. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 62.5pg/mL-4000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Cathepsin L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Cathepsin L standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: E113-V333

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Cathepsin L

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Alternative Name: Cathepsin L

Background:

Synonyms: Cathepsin L1, Cathepsin L1 light chain, CathepsinL, CATL, CATL1_HUMAN, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP

Background: Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.

Gene ID: 1514

NCBI Accession: NP_001244900

UniProt: P07711

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades