Corin ELISA Kit

Catalog No. ABIN6720220

Supplier Product No.: koa0759

Human Corin ELISA Kit Colorimetric assay for quantification of Human Corin in Cell Culture Supernatant, Plasma (EDTA - heparin) and Serum

Quick Overview for Corin ELISA Kit (ABIN6720220)

Target: Corin (CORIN) (Corin, Serine Peptidase (CORIN))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details Corin ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Supplier Product No.: koa0759

Supplier: Rockland

Purpose: Human Corin Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human Corin. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Corin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. It is recommended that each human Corin standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: S67-N1042

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Corin

Target: Corin (CORIN) (Corin, Serine Peptidase (CORIN))

Alternative Name: Corin

Background:

Synonyms: activated protease fragment, ATC 2, ATC2, Atrial natriuretic peptide-converting enzyme, Atrial natriuteric peptide converting enzyme, Corin, Corin serine peptidase, CORIN_HUMAN, CRN, Heart specific serine proteinase, Heart specific serine proteinase ATC2, Heart-specific serine proteinase ATC2, Lrp 4, Lrp4, MGC119742, Pro ANP convertase, Pro ANP converting enzyme, Pro-ANP-converting enzyme, TMPRSS 10, TMPRSS10, Transmembrane protease serine 10

Background: Atrial natriuretic peptide-converting enzyme, also called CRN or ATC2, is a protein that in humans is encoded by the CORIN gene. It is mapped to 4p12. This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. It has been found that Corin is the pro-ANP-converting enzyme and that Corin-mediated pro-ANP activation may play a role in regulating blood pressure. What's more, Corin and ANP are essential for physiologic changes at the maternal-fetal interface. This protein may also function as a pro-brain-type natriuretic peptide convertase. Corin gene mutations which cause Corin to impair activity might be an underlying mechanism in hypertension.

Gene ID: 10699

NCBI Accession: NP_001265514

UniProt: Q9Y5Q5

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones