CXCL11 ELISA Kit

Catalog No. ABIN6720222

Supplier Product No.: koa0431

This Human CXCL11 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CXCL11.

Quick Overview for CXCL11 ELISA Kit (ABIN6720222)

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CXCL11 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Supplier Product No.: koa0431

Supplier: Rockland

Purpose: Human CXCL11 - I-TAC Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CXCL11. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CXCL11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CXCL11 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: F22-F94

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CXCL11

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Alternative Name: CXCL11/I-TAC

Background:

Synonyms: b R1|Beta R1, C X C motif chemokine 11, C-X-C motif chemokine 11, Chemokine C-X-C motif ligand 11, CXL11_HUMAN, H174, I TAC, Interferon gamma inducible protein 9, Interferon inducible T cell a chemoattractant I TAC, Interferon inducible T cell alpha chemoattractant, IP9, ITAC, SCYB11, SCYB9B, Small inducible cytokine B11, Small inducible cytokine subfamily B member 11, Small inducible cytokine subfamily B member 9B, Small inducible cytokine subfamily B member 11, Small inducible cytokine subfamily B member 9B

Background: Chemokine(C-X-C motif) ligand 11(CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant(I-TAC) and Interferon-gamma-inducible protein 9(IP-9). By PCR analysis of somatic cell hybrids, the CXCL11 gene was mapped to chromosome 4, and using FISH, it was located to 4q21.2. The CXCL11 was strongly induced by IFNG and beta-interferon, but only weakly induced by alpha-interferon. Induction of CXCL11 required expression of STAT1. What's more, it found that monocyte chemotaxis occurs in response to recombinant CXCL11. RT-PCR analysis detected CXCL11 expression in brain tissue from a patient with AIDS dementia and a patient with multiple sclerosis but not in 2 controls.

Gene ID: 6373

NCBI Accession: NP_001289052

UniProt: O14625