CXCL14 ELISA Kit

Catalog No. ABIN6720223

Supplier Product No.: koa0761

Human CXCL14 ELISA Kit Colorimetric assay for quantification of Human CXCL14 in Cell Culture Supernatant, Plasma (EDTA - heparin) and Serum

Quick Overview for CXCL14 ELISA Kit (ABIN6720223)

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CXCL14 ELISA Kit

Minimum Detection Limit: 125 pg/mL

Supplier Product No.: koa0761

Supplier: Rockland

Purpose: Human CXCL14 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CXCL14. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 62.5pg/mL-4000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CXCL14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CXCL14 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: S35-E111

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CXCL14

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Alternative Name: CXCL14

Background:

Synonyms: BMAC, bolekine, BRAK, C-X-C motif chemokine 14, Chaemokine CXC motif ligand 14, Chemokine BRAK, CXC chemokine in breast and kidney, CXL14_HUMAN, JSC, Kec, Kidney-expressed chemokine CXC, KS1, MGC10687, MGC124510, MGC90667, MIP 2 gamma, MIP-2G, MIP2G, MIP2gamma, NJAC, PRO273, PSEC0212, Scyb14, Small Inducible Cytokine B14, Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK), Small Inducible Cytokine subfamily B member 14, Small-inducible cytokine B14

Background: Chemokine(C-X-C motif) ligand 14(CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK(for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.

Gene ID: 9547

NCBI Accession: NP_004878

UniProt: O95715

Pathways: Autophagy