FSTL3 ELISA Kit

Catalog No. ABIN6720277

Product Details FSTL3 ELISA Kit

Target: FSTL3 (Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: FLRG, follistatin-like 3, follistatin-like 3 glycoprotein, Follistatin-like protein 3, Follistatin-related gene protein, Follistatin-related protein 3, FSRP, Fstl3, FSTL3_HUMAN

Background: FSTL3(Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins thought to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. The FSTL3 gene was localized to chromosome 19p13. This gene involved in hematopoiesis and in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. It may have a role in leukemogenesis.

Gene Name: FSTL3

Production: Natural and recombinant human FLRG. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: M27-V263

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human FLRG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), urine or human milk to each empty well. It is recommended that each human FLRG standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for FSTL3

Target: FSTL3 (Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3))

Alternative Name: FLRG - FSTL3 (FSTL3 Products)

Synonyms: E030038F23Rik ELISA Kit, Flrg ELISA Kit, FLRG ELISA Kit, FSRP ELISA Kit, follistatin-like 3 ELISA Kit, follistatin like 3 ELISA Kit, Fstl3 ELISA Kit, FSTL3 ELISA Kit

Gene ID: 10272

NCBI Accession: NP_005851

UniProt: O95633