IGF2 ELISA Kit

Catalog No. ABIN6720286

Supplier Product No.: koa0197

Rat IGF2 ELISA Kit Colorimetric assay for quantification of Rat IGF2.

Quick Overview for IGF2 ELISA Kit (ABIN6720286)

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details IGF2 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Supplier Product No.: koa0197

Supplier: Rockland

Purpose: Rat IGF-2 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant rat IGF-2. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 5 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat IGF-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat IGF-2 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: A25-E91

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IGF2

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Alternative Name: IGF-2

Background:

Synonyms: C11orf43, FLJ22066, FLJ44734, IGF 2, IGF II, IGF-II, IGF2, IGF2_HUMAN, IGFII, INSIGF, Insulin like Growth Factor 2, Insulin like growth factor 2 (somatomedin A), Insulin like growth factor II, Insulin like growth factor II precursor, Insulin like growth factor type 2, pp9974, Preptin, Putative insulin like growth factor II associated protein, Somatomedin A, Somatomedin-A

Background: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis, prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.

Gene ID: 24483

NCBI Accession: NP_001177091

UniProt: P01346

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation