CCL20 ELISA Kit

Catalog No. ABIN6720291

Supplier Product No.: koa0263

This Rat CCL20 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat CCL20.

Quick Overview for CCL20 ELISA Kit (ABIN6720291)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 pg/mL - 500 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CCL20 ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Supplier Product No.: koa0263

Supplier: Rockland

Purpose: Rat MIP-3 alpha - CCL20 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant rat MIP-3 alpha. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL rat MIP-3 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat MIP-3 alpha standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence:  S27-M96

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: MIP-3 alpha/CCL20

Background:

Synonyms: Beta chemokine exodus 1, Beta-chemokine exodus-1, C C motif chemokine 20, CC chemokine LARC, Ccl20, CCL20(2-70), CCL20_HUMAN, Chemokine (C C motif) ligand 20, CKb4, Exodus 1, LARC, Liver and activation regulated chemokine, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP 3 alpha, MIP 3a, MIP-3-alpha, MIP3 alpha, MIP3A, MIP3alpha, SCYA20, Small inducible cytokine A20, Small inducible cytokine subfamily A (Cys Cys) member 20, Small-inducible cytokine A20, ST38

Background: Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Gene ID: 29538

NCBI Accession: NP_062106

UniProt: P97884

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection