CD56 ELISA Kit

Catalog No. ABIN6720296

Supplier Product No.: koa0716

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse CD56. There is 1 publication available.

Quick Overview for CD56 ELISA Kit (ABIN6720296)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 625 pg/mL - 40000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CD56 ELISA Kit

Minimum Detection Limit: 625 pg/mL

Supplier Product No.: koa0716

Supplier: Rockland

Purpose: Mouse CD56 - NCAM1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse CD56. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 20 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 625pg/mL-40,000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL mouse CD56 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD56 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: L20-T711

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for CD56 ELISA Kit (ABIN6720296)

Kagiava, Karaiskos, Richter, Tryfonos, Jennings, Heslegrave, Sargiannidou, Stavrou, Zetterberg, Reilly, Christodoulou, Horvath, Kleopa: "AAV9-mediated Schwann cell-targeted gene therapy rescues a model of demyelinating neuropathy." in: Gene therapy, Vol. 28, Issue 10-11, pp. 659-675, (2022) (PubMed).

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: CD56/NCAM1

Background:

Synonyms: CD56, N-CAM-1, NCAM-1, NCAM1, NCAM1_HUMAN, Neural cell adhesion molecule 1

Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Gene ID: 17967

NCBI Accession: NP_001074914

UniProt: P13595