FCN1 ELISA Kit

Catalog No. ABIN6720297

Supplier Product No.: koa0741

This Human FCN1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human FCN1.

Quick Overview for FCN1 ELISA Kit (ABIN6720297)

Target: FCN1 (Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Product Details FCN1 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Supplier Product No.: koa0741

Supplier: Rockland

Purpose: Human Ficolin-1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human Ficolin-1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 20 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Ficolin-1 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: A30-A326

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for FCN1

Target: FCN1 (Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1))

Alternative Name: Ficolin-1

Background:

Synonyms: Collagen/fibrinogen domain containing protein 1, Collagen/fibrinogen domain-containing protein 1, Fcn1, FCN1_HUMAN, FCNM, ficolin (collagen/fibrinogen domain containing) 1, Ficolin 1, Ficolin A, Ficolin alpha, Ficolin-1, Ficolin-A, Ficolin-alpha, M ficolin, M-ficolin

Background: Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.

Gene ID: 2219

NCBI Accession: NP_001994

UniProt: O00602

Pathways: Complement System