ADAM9 ELISA Kit

Catalog No. ABIN6720308

Supplier Product No.: koa0806

This Colorimetric ELISA kit is designed for the quantitative measurement of Human ADAM9.

Quick Overview for ADAM9 ELISA Kit (ABIN6720308)

Target: ADAM9 (ADAM Metallopeptidase Domain 9 (ADAM9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Product Details ADAM9 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Supplier Product No.: koa0806

Supplier: Rockland

Purpose: Human ADAM9 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human ADAM9. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human ADAM9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human ADAM9 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: A29-D697

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for ADAM9

Target: ADAM9 (ADAM Metallopeptidase Domain 9 (ADAM9))

Alternative Name: ADAM9

Background:

Synonyms: ADAM 9, ADAM metallopeptidase domain 9, Adam9, ADAM9_HUMAN, Cellular disintegrin-related protein, Cone rod dystrophy 9, CORD9, Disintegrin and metalloproteinase domain-containing protein 9, MCMP, MDC9, Meltrin-gamma, Metalloprotease/disintegrin/cysteine-rich protein 9, Mltng, Myeloma cell metalloproteinase

Background: Disintegrin and metalloproteinase domain-containing protein 9, also known as MCMP or MDC9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM('a disintegrin and metalloproteinase') family and exhibits a number of characteristics of class III reprolysins. This gene is mapped to 8p11.22. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.

Gene ID: 8754

NCBI Accession: NP_003807

UniProt: Q13443

Pathways: Cellular Response to Molecule of Bacterial Origin, SARS-CoV-2 Protein Interactome