Aggrecan ELISA Kit

Catalog No. ABIN6720312

Supplier Product No.: koa0548

This Human Aggrecan ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Aggrecan.

Quick Overview for Aggrecan ELISA Kit (ABIN6720312)

Target: Aggrecan (ACAN)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Urine

Product Details Aggrecan ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Supplier Product No.: koa0548

Supplier: Rockland

Purpose: Human Aggrecan Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human Aggrecan. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 50 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Aggrecan standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or urine to each empty well. It is recommended that each human Aggrecan standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: V20-G675

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Aggrecan

Target: Aggrecan (ACAN)

Alternative Name: Aggrecan

Background:

Synonyms: ACAN, AGC1, AGCAN, Aggrecan 1, Aggrecan core protein, Aggrecan proteoglycan, Aggrecan structural proteoglycan of cartilage, Aggrecan1, ATEGQV, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122, Chondroitin sulfate proteoglycan core protein 1, CSPG1, CSPGCP, JSCATE, Large aggregating proteoglycan, mcspg, mgsk16, MSK16, SEDK

Background: Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.

Gene ID: 176

UniProt: P16112

Pathways: Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport