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Baculoviral IAP Repeat-Containing 7 (BIRC7) ELISA Kit ELISA Kit

BIRC7 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Lysate
Catalog No. ABIN6720313
$459.00
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1 kit
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  • Target
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.78 ng/mL - 50 ng/mL
    Minimum Detection Limit
    0.78 ng/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: Baculoviral IAP repeat containing 7, Baculoviral IAP repeat containing protein 7, Baculoviral IAP repeat-containing protein 7, BIRC 7, BIRC7, BIRC7_HUMAN, KIAP, Kidney inhibitor of apoptosis protein, LIVIN, Livin inhibitor of apotosis, Melanoma inhibitor of apoptosis protein, ML IAP, ML-IAP, MLIAP, RING finger protein 50, RNF 50, RNF50

    Background: BIRC7, also called KIAP or Livin, is a protein that in humans is encoded by the BIRC7 gene. This gene is mapped to 20q13.33. It encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. BIRC7 blocked apoptosis induced by BAX induction of cytochrome C release from mitochondria, and it also can block apoptosis induced by chemotherapeutic agents.

    Gene Name: BIRC7

    Production: Natural and recombinant human Livin. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: M1-S298

  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Livin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, cell lysates or tissue homogenates to each empty well. It is recommended that each human Livin standard solution and each sample be measured in duplicate.

    ELISA Dilution: 0.78 ng/mL-50 ng/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target
    Alternative Name
    Livin (BIRC7 ELISA Kit Abstract)
    Gene ID
    79444
    NCBI Accession
    NP_071444
    UniProt
    Q96CA5
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