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BIRC7 ELISA Kit (Baculoviral IAP Repeat-Containing 7) ELISA Kit

BIRC7 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN625464
$546.70
Plus shipping costs $45.00
96 tests
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  • Target
    BIRC7
    Reactivity
    Human
    • 5
    • 3
    • 2
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Livin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human Livin.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling BIRC7 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target
    BIRC7
    Alternative Name
    Livin (BIRC7 ELISA Kit Abstract)
    Synonyms
    KIAP, LIVIN, ML-IAP, MLIAP, RNF50, EIAP/XLX, XLX, kiap, livin, ml-iap, mliap, rnf50, xEIAP, RGD1562883, zgc:165605, E130019N06, Livin, birc7, bird7a, xEIAP/XLX, xlx, baculoviral IAP repeat containing 7, baculoviral IAP repeat-containing 7, baculoviral IAP repeat-containing 7 (livin), baculoviral IAP repeat containing 7 S homeolog, BIRC7, birc7, Birc7, birc7.S
    Gene ID
    79444
    UniProt
    Q96CA5
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