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BIRC7 ELISA Kit

BIRC7 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4883624
  • Target See all BIRC7 ELISA Kits
    BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))
    Reactivity
    • 5
    • 3
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Livin ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human Livin.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all BIRC7 ELISA Kits
    BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))
    Alternative Name
    Livin (BIRC7 Products)
    Synonyms
    KIAP ELISA Kit, LIVIN ELISA Kit, ML-IAP ELISA Kit, MLIAP ELISA Kit, RNF50 ELISA Kit, EIAP/XLX ELISA Kit, XLX ELISA Kit, kiap ELISA Kit, livin ELISA Kit, ml-iap ELISA Kit, mliap ELISA Kit, rnf50 ELISA Kit, xEIAP ELISA Kit, RGD1562883 ELISA Kit, zgc:165605 ELISA Kit, E130019N06 ELISA Kit, Livin ELISA Kit, birc7 ELISA Kit, bird7a ELISA Kit, xEIAP/XLX ELISA Kit, xlx ELISA Kit, EIAP/XLX-B ELISA Kit, birc7b ELISA Kit, baculoviral IAP repeat containing 7 ELISA Kit, baculoviral IAP repeat-containing 7 ELISA Kit, baculoviral IAP repeat-containing 7 (livin) ELISA Kit, baculoviral IAP repeat containing 7 S homeolog ELISA Kit, baculoviral IAP repeat containing 7 L homeolog ELISA Kit, BIRC7 ELISA Kit, birc7 ELISA Kit, Birc7 ELISA Kit, birc7.S ELISA Kit, birc7.L ELISA Kit
    Gene ID
    79444
    UniProt
    Q96CA5
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