PTX3 ELISA Kit

Catalog No. ABIN6720329

Supplier Product No.: koa0521

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PTX3.

Quick Overview for PTX3 ELISA Kit (ABIN6720329)

Target: PTX3 (Pentraxin 3 (PTX3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Saliva, Serum

Product Details PTX3 ELISA Kit

Minimum Detection Limit: 312 pg/mL

Supplier Product No.: koa0521

Supplier: Rockland

Purpose: Human PTX3 - Pentraxin 3 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human PTX3. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 312pg/mL-20000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human PTX3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(EDTA) or saliva to each empty well. It is recommended that each human PTX3 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: E18-S381

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for PTX3

Target: PTX3 (Pentraxin 3 (PTX3))

Alternative Name: PTX3/Pentraxin 3

Background:

Synonyms: AI607804, Pentaxin 3, Pentaxin-related protein PTX3, Pentraxin 3 long, Pentraxin-related gene, Pentraxin-related gene rapidly induced by IL-1 beta, Pentraxin-related protein PTX3, PTX3, PTX3_HUMAN, TNF alpha-induced protein 5, TNFAIP5, TSG-14, TSG14, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, Tumor necrosis factor-inducible protein TSG-14

Background: PTX3(Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complement activation and facilitates pathogen recognition by macrophages and DCs.

Gene ID: 5806

NCBI Accession: NP_002843

UniProt: P26022