APOE ELISA Kit

Catalog No. ABIN6720363

Supplier Product No.: koa0878

This Human APOE ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human APOE.

Quick Overview for APOE ELISA Kit (ABIN6720363)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Milk, Plasma (EDTA - heparin), Saliva, Serum, Urine

Product Details APOE ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Supplier Product No.: koa0878

Supplier: Rockland

Purpose: Human APOE Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural human APOE. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 50 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL human APOE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, milk or urine to each empty well. It is recommended that each human APOE standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: K19-H317

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: APOE

Background:

Synonyms: AD2, Alzheimer disease 2, Apo E, Apo-E, ApoE, APOEA, Apolipoprotein E, Apolipoprotein-E, ApolipoproteinE, Apoprotein, LDLCQ5, LPG

Background: Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimer's disease and cardiovascular disease.

Gene ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism