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TGFB1 ELISA Kit (Transforming Growth Factor, beta 1) ELISA Kit

TGFB1 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Pubmed (20)
Catalog No. ABIN6730882
$513.68
Plus shipping costs $45.00
96 tests
local_shipping Shipping to: United States
Delivery in 9 to 11 Business Days
  • Target
    TGFB1
    Reactivity
    • 14
    • 12
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TGFb1 in human serum, platelet-poor plasma, tissue homogenates, cell culture supernates.

    We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
    Sample Type
    Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1)
    Cross-Reactivity (Details)
    No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.
    Sensitivity
    5.7 pg/mL
    Components
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
  • Comment

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Sample Volume
    100 μL
    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards,
    2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
    4. Aspirate and wash 3 times,
    5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    6. Aspirate and wash 5 times,
    7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    8. Add 50μL Stop Solution. Read at 450nm immediately.
    Reagent Preparation
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 1,000pg/mL. Prepare 7 tubes containing 0.5 mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    6. Contaminated water or container for reagent preparation will influence the detection result.
    Sample Preparation
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV < 10%
    Inter-Assay: CV < 12%
    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Storage
    4 °C/-20 °C
    Storage Comment
    1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
    2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
    .
    Expiry Date
    6 months
  • Cen, Liu, Wang, Yang, Shi, Liang: "Glucosamine oral administration as an adjunct to hyaluronic acid injection in treating temporomandibular joint osteoarthritis." in: Oral diseases, Vol. 24, Issue 3, pp. 404-411, 2018 (PubMed).

    Sun, Xiu, Wang, Brigstock, Li, Qu, Gao: "Lipopolysaccharide enhances TGF-β1 signalling pathway and rat pancreatic fibrosis." in: Journal of cellular and molecular medicine, Vol. 22, Issue 4, pp. 2346-2356, 2018 (PubMed).

    Zhang, Che, Yang, Chi, Meng, Shen, Qi, Liu, Lv, Li, Meng, Liu, Shang, Yu: "Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancer." in: Oncotarget, Vol. 8, Issue 59, pp. 99801-99815, 2017 (PubMed).

    Mateu-Jimenez, Curull, Pijuan, Sánchez-Font, Rivera-Ramos, Rodríguez-Fuster, Aguiló, Gea, Barreiro: "Systemic and Tumor Th1 and Th2 Inflammatory Profile and Macrophages in Lung Cancer: Influence of Underlying Chronic Respiratory Disease." in: Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, Vol. 12, Issue 2, pp. 235-248, 2017 (PubMed).

    Zhang, Abudula, Awuti, Wang, Aihemaiti, Tusung, Sulaiman, Upur: "Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription." in: Experimental and therapeutic medicine, Vol. 14, Issue 1, pp. 267-275, 2017 (PubMed).

    Jiang, Wang, Tang, Yao, Zhou: "Regulation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells." in: COPD, pp. 1-6, 2016 (PubMed).

    Salehi, Doosti, Beheshti, Janzamin, Sahraian, Izad: "Differential Frequency of CD8+ T Cell Subsets in Multiple Sclerosis Patients with Various Clinical Patterns." in: PLoS ONE, Vol. 11, Issue 7, pp. e0159565, 2016 (PubMed).

    Pandey, Ali, Mohammad, Pasha: "Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population." in: Therapeutics and clinical risk management, Vol. 12, pp. 1207-21, 2016 (PubMed).

    Samuel, Ahmad, Ramasamy, Karunanithi, Naveen, Murali, Abbas, Kamarul: "Platelet-rich concentrate in serum free medium enhances osteogenic differentiation of bone marrow-derived human mesenchymal stromal cells." in: PeerJ, Vol. 4, pp. e2347, 2016 (PubMed).

    Zhao, Zhu, Zhang, Han, Gao, Wei, Xie, Li, Sun, Wang, Guo: "Gemcitabine treatment enhanced the anti-tumor effect of cytokine induced killer cells by depletion of CD4(+)CD25(bri) regulatory T cells." in: Immunology letters, Vol. 181, pp. 36-44, 2016 (PubMed).

    Uluçay, Çam, Bat?r, Sütçü, Bayturan, Demircan: "A novel association between TGFb1 and ADAMTS4 in coronary artery disease: A new potential mechanism in the progression of atherosclerosis and diabetes." in: Anadolu kardiyoloji dergisi : AKD = the Anatolian journal of cardiology, 2015 (PubMed).

    Rajesh, Prajapati: "A copper-catalyzed one-pot, three-component tandem conjugative alkynylation/6-endo cyclization sequence: access to pyrano[2,3-d]-pyrimidines." in: Organic & biomolecular chemistry, Vol. 13, Issue 16, pp. 4668-72, 2015 (PubMed).

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    Salamanna, Pagani, Maglio, Borsari, Giavaresi, Martelli, Buontempo, Fini: "Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells." in: Histology and histopathology, Vol. 31, Issue 1, pp. 83-93, 2015 (PubMed).

    Mat?j, Sm?táková, Vašáková, Nováková, Sterclová, Kukal, Olejár: "PAR-2, IL-4R, TGF-? and TNF-? in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosis." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 533-538, 2014 (PubMed).

    Vasakova, Sterclova, Stranska, Mandakova, Skibova, Matej: "Biomarkers of fibroproliferative healing in fibrosing idiopathic interstitial pneumonias." in: The open respiratory medicine journal, Vol. 6, pp. 160-4, 2013 (PubMed).

    Mern, Fontana, Beierfuß, Thomé, Hegewald et al.: "A combinatorial relative mass value evaluation of endogenous bioactive proteins in three-dimensional cultured nucleus pulposus cells of herniated intervertebral discs: identification of potential ..." in: PLoS ONE, Vol. 8, Issue 11, pp. e81467, 2013 (PubMed).

    Wang, Liu, Peng, Tang, Tang, Chen, Lou, Zhang: "Mesangial cells stimulated by immunoglobin A1 from IgA nephropathy upregulates transforming growth factor-beta1 synthesis in podocytes via renin-angiotensin system activation." in: Archives of medical research, Vol. 41, Issue 4, pp. 255-60, 2010 (PubMed).

    Zhang, Long, Sun, Wang, Li, Wu, Guo, Li, Niu, Li, Liu, Mei et al.: "Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by ..." in: European journal of pharmacology, Vol. 651, Issue 1-3, pp. 187-96, 2010 (PubMed).

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  • Target
    TGFB1
    Alternative Name
    Transforming Growth Factor Beta 1 (TGFb1) (TGFB1 ELISA Kit Abstract)
    Synonyms
    CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta, TGF-BETA-1, TGF-beta5, ced, dpd1, lap, tgf-beta, tgfb, tgfb5, tgfbeta, TGF-beta1, TGFbeta1, Tgfb, Tgfb-1, ai39657, tgfb1, wu:fb13a07, xx:ai39657, TGFB1, csd, cdb1, cdg2, csd1, csd2, csd3, ebmd, lcd1, bigh3, cdgg1, betaig-h3, TGFB4, transforming growth factor beta 1, transforming growth factor beta-1, transforming growth factor beta 1 L homeolog, transforming growth factor, beta 1, transforming growth factor, beta 1a, transforming growth factor beta induced L homeolog, TGFB1, Tgfb1, tgfb1.L, tgfb1a, tgfbi.L
    UniProt
    P01137
    Pathways
    EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints
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