TGFB1 ELISA Kit

Catalog No. ABIN6973704

Product Details TGFB1 ELISA Kit

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Chicken

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Purpose: For the quantitative determination of chicken transforming growth factor β1 (TGF-β1) concentrations in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of chicken TGF-β1. No significant cross-reactivity or interference between chicken TGF-β1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between chicken TGF-β1 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.078 ng/mL

Components:

• Assay plate
• Standard
• HRP-avidin (100 x concentrate)
• Biotin-antibody (100 x concentrate)
• Sample Diluent
• HRP-avidin Diluent
• Biotin-antibody Diluent
• Wash Buffer (25 x concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol:

1. Prepare reagents, samples and standards as instructed.
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
3. Remove the liquid of each well, don't wash.
4. Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
5. Aspirate and wash 3 times.
6. Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
7. Aspirate and wash 5 times.
8. Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
9. Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.

Reagent Preparation:

1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
3. Wash Buffer (1x) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 20 ng/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (20 ng/mL). Sample Diluent serves as the zero standard (0 ng/mL).

Note:

• Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
• Bring all reagents to room temperature (18-25 °C) before use for 30 min.
• Prepare fresh standard for each assay. Use within 4 hours and discard after use.
• Making serial dilution in the wells directly is not permitted.
• Please carefully reconstitute Standards according to the i nstruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
• Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.

Sample Preparation:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Assay Precision: Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.

Expiry Date: 6 months

References for TGFB1 ELISA Kit (ABIN6973704)

Huang, Zhang, Li, Song, Yan, Xu, Li: "Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge." in: Parasitology international, Vol. 64, Issue 5, pp. 408-16, (2015) (PubMed).

Huang, Zhang, Li, Song, Yan, Xu, Li: "Immune protection of microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens." in: Avian pathology : journal of the W.V.P.A, Vol. 44, Issue 5, pp. 392-400, (2015) (PubMed).

Nirala, Gohil: "Effect of garlic component s-allyl cysteine sulfoxide on glycated human serum albumin induced activation of endothelial cells: an in vitro study." in: European review for medical and pharmacological sciences, Vol. 19, Issue 11, pp. 2125-31, (2015) (PubMed).

Zhang, Huang, Li, Sui, Wang, Liu, Xu, Yan, Song, Li: "Identification and Molecular Characterization of Microneme 5 of Eimeria acervulina." in: PLoS ONE, Vol. 9, Issue 12, pp. e115411, (2014) (PubMed).

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: transforming growth factor, beta 1 (TGFB1 Products)

Synonyms: CED ELISA Kit, DPD1 ELISA Kit, LAP ELISA Kit, TGFB ELISA Kit, TGFbeta ELISA Kit, TGF-beta ELISA Kit, TGF-BETA-1 ELISA Kit, TGF-beta5 ELISA Kit, ced ELISA Kit, dpd1 ELISA Kit, lap ELISA Kit, tgf-beta ELISA Kit, tgfb ELISA Kit, tgfb5 ELISA Kit, tgfbeta ELISA Kit, TGF-beta1 ELISA Kit, TGFbeta1 ELISA Kit, Tgfb ELISA Kit, Tgfb-1 ELISA Kit, ai39657 ELISA Kit, tgfb1 ELISA Kit, wu:fb13a07 ELISA Kit, xx:ai39657 ELISA Kit, TGFB1 ELISA Kit, csd ELISA Kit, cdb1 ELISA Kit, cdg2 ELISA Kit, csd1 ELISA Kit, csd2 ELISA Kit, csd3 ELISA Kit, ebmd ELISA Kit, lcd1 ELISA Kit, bigh3 ELISA Kit, cdgg1 ELISA Kit, betaig-h3 ELISA Kit, TGFB4 ELISA Kit, transforming growth factor beta 1 ELISA Kit, transforming growth factor beta-1 ELISA Kit, transforming growth factor beta 1 L homeolog ELISA Kit, transforming growth factor, beta 1 ELISA Kit, transforming growth factor, beta 1a ELISA Kit, transforming growth factor beta induced L homeolog ELISA Kit, TGFB1 ELISA Kit, Tgfb1 ELISA Kit, tgfb1.L ELISA Kit, tgfb1a ELISA Kit, tgfbi.L ELISA Kit

Background:

Abbreviation: TGFB1

Alias: CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 protein|latency-associated peptide, transforming growth factor-beta1

UniProt: P09531

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints