TGFB1 ELISA Kit

Catalog No. ABIN411355

Mouse TGFB1 ELISA Kit, Colorimetric assay for quantification of Mouse TGFB1, that has been mentioned in 29+ publications.

Quick Overview for TGFB1 ELISA Kit (ABIN411355)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Binding Specificity: AA 279-390

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (EDTA), Urine

Product Details TGFB1 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Mouse TGF Beta 1 ELISA Kit to quantitate Mouse Tgfb1 in cell culture supernatants, serum, plasma (EDTA) and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: A279-S390

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.1%, 6.9%, 7.1%
Inter-Assay Precision (CV%): 4.7%, 7.1%, 7.1%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for TGFB1 ELISA Kit (ABIN411355)

Denslow, Switalska, Nowak, Maciejewska, Chlopicki, Marcinek, Gebicki, Wietrzyk: "The effects of 1,4-dimethylpyridine in metastatic prostate cancer in mice." in: BMC cancer, Vol. 17, Issue 1, pp. 177, (2018) (PubMed).

Shen, Zhang, Li, Liu, Yu, Hu, Hui, Guo, Zhang: "Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer." in: Oncotarget, Vol. 8, Issue 30, pp. 49395-49408, (2018) (PubMed).

Li, Liu, Duan, Liu, Wang, Lian, Zhuang, Fan: "Combined Blockade of T Cell Immunoglobulin and Mucin Domain 3 and Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Results in Durable Therapeutic Efficacy in Mice with Intracranial Gliomas." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 3593-3602, (2018) (PubMed).

Yang, Zhang, Cai, Shang: "Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma." in: International journal of molecular medicine, Vol. 41, Issue 2, pp. 818-828, (2018) (PubMed).

Wang, Liu, Wang, Yang, Li, Ding, Sun, Bai, Li: "Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 2305683, (2018) (PubMed).

Li, Peng, Sun, Liu, Peng, Zhang: "LincRNA 1700020I14Rik alleviates cell proliferation and fibrosis in diabetic nephropathy via miR-34a-5p/Sirt1/HIF-1α signaling." in: Cell death & disease, Vol. 9, Issue 5, pp. 461, (2018) (PubMed).

Xu, Liang, Li, Ma: "Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer." in: Oncology letters, Vol. 15, Issue 6, pp. 9293-9298, (2018) (PubMed).

Kabel, Elkhoely: "Targeting proinflammatory cytokines, oxidative stress, TGF-β1 and STAT-3 by rosuvastatin and ubiquinone to ameliorate trastuzumab cardiotoxicity." in: Biomedicine & pharmacotherapy, Vol. 93, pp. 17-26, (2017) (PubMed).

Chen, Zeng, Zhan, Wang, Jiang, Li: "Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-mediated paracrine Wnt10b." in: Oncogene, Vol. 36, Issue 33, pp. 4692-4705, (2017) (PubMed).

Zhang, Meng, Jia, Bu, Ren, Zhang, Tang: "Herbal Compound Songyou Yin and Moderate Swimming Suppress Growth and Metastasis of Liver Cancer by Enhancing Immune Function." in: Integrative cancer therapies, Vol. 15, Issue 3, pp. 368-75, (2017) (PubMed).

Wang, Zhang, Wang, Gadahi, Xu, Yan, Song, Li: "Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1α) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis." in: Frontiers in microbiology, Vol. 8, pp. 168, (2017) (PubMed).

Liu, Ma, Sun, Lu, Ehsan, Hasan, Xu, Yan, Song, Li: "Effects of Recombinant Toxoplasma gondii Citrate Synthase I on the Cellular Functions of Murine Macrophages In vitro." in: Frontiers in microbiology, Vol. 8, pp. 1376, (2017) (PubMed).

Li, Ma, Zha, Quan, Pan, Sun, Hu, Zhao: "Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by γ-ray irradiation in mice." in: Oncotarget, Vol. 8, Issue 37, pp. 60789-60808, (2017) (PubMed).

Wang, Zhang, Wang, Gadahi, Xie, Xu, Yan, Song, Li: "Toxoplasma gondii excretory/secretory antigens (TgESAs) suppress pro-inflammatory cytokine secretion by inhibiting TLR-induced NF-κB activation in LPS-stimulated murine macrophages." in: Oncotarget, Vol. 8, Issue 51, pp. 88351-88359, (2017) (PubMed).

Denslow, Świtalska, Jarosz, Papiernik, Porshneva, Nowak, Wietrzyk: "Clopidogrel in a combined therapy with anticancer drugs-effect on tumor growth, metastasis, and treatment toxicity: Studies in animal models." in: PLoS ONE, Vol. 12, Issue 12, pp. e0188740, (2017) (PubMed).

Zhou, Wang, Wang, Liu, Zhang, Yin, Wang, Kang, Hou: "GHK Peptide Inhibits Bleomycin-Induced Pulmonary Fibrosis in Mice by Suppressing TGFβ1/Smad-Mediated Epithelial-to-Mesenchymal Transition." in: Frontiers in pharmacology, Vol. 8, pp. 904, (2017) (PubMed).

Wang, Wang, Sun, Zhang, Liu, Gadahi, Hassan, Xu, Yan, Song, Li: "Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha." in: BMC infectious diseases, Vol. 15, pp. 448, (2016) (PubMed).

Qin, Lu, Guo, Luo, Hu, Huang, Chen, Wang, Ma, Yang, Jiang, Zhou: "A deleterious role for Th9/IL-9 in hepatic fibrogenesis." in: Scientific reports, Vol. 6, pp. 18694, (2016) (PubMed).

Lu, Cao, Wang, Zheng, Hou, Zhu, Hu: "Mature dendritic cells cause Th17/Treg imbalance by secreting TGF-β1 and IL-6 in the pathogenesis of experimental autoimmune encephalomyelitis." in: Central-European journal of immunology, Vol. 41, Issue 2, pp. 143-52, (2016) (PubMed).

Wang, Jinnin, Kobayashi, Kudo, Inoue, Nakayama, Honda, Makino, Kajihara, Makino, Fukushima, Inagaki, Ihn: "Mice overexpressing integrin ?v in fibroblasts exhibit dermal thinning of the skin." in: Journal of dermatological science, Vol. 79, Issue 3, pp. 268-78, (2015) (PubMed).

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1

Background:

Background: Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative ion of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.

Gene Full Name: transforming growth factor, beta 1

Gene ID: 21803

UniProt: P04202

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints