TGFB1 ELISA Kit

Catalog No. ABIN411353

This Colorimetric ELISA kit is designed for the quantitative measurement of Human TGFB1. There are 25 publications available.

Quick Overview for TGFB1 ELISA Kit (ABIN411353)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Binding Specificity: AA 279-390

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (EDTA), Urine

Product Details TGFB1 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Human TGF Beta 1 ELISA Kit to quantitate Human TGFB1 in cell culture supernatants, serum, plasma s (EDTA) and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: A279-S390

Capture antibody and Detection antibody: polyclonal antibody from chicken, polyclonal antibody from chicken

Cross-Reactivity (Details): There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5 <1 % .

Sensitivity: < 1 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.6%, 5.4%, 7%
Inter-Assay Precision (CV%): 6.2%, 5.4%, 7.1%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for TGFB1 ELISA Kit (ABIN411353)

Li, Wang, Wang, Sun, Cai, Liu, Zhang, Lu, Li, Chen, Chen, Jiang: "Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells." in: Oncotarget, Vol. 8, Issue 16, pp. 26256-26268, (2018) (PubMed).

Liu, Zhen, Song, Chen, Sun, Li, Zhou, Yan, Ding, Sun: "Low-dose lymphocyte immunotherapy rebalances the peripheral blood Th1/Th2/Treg paradigm in patients with unexplained recurrent miscarriage." in: Reproductive biology and endocrinology : RB&E, Vol. 15, Issue 1, pp. 95, (2018) (PubMed).

Zhu, Gu, Ma, Gao, Liu, Xiao, Wang, Chen, Li: "Biomarkers for Pulmonary Inflammation and Fibrosis and Lung Ventilation Function in Chinese Occupational Refractory Ceramic Fibers-Exposed Workers." in: International journal of environmental research and public health, Vol. 15, Issue 1, (2018) (PubMed).

Jiang, Tan, Cai, Zhao, Mao, Zhang, Xu, Yan, Qian, Yan: "Human Umbilical Cord MSC-Derived Exosomes Suppress the Development of CCl4-Induced Liver Injury through Antioxidant Effect." in: Stem cells international, Vol. 2018, pp. 6079642, (2018) (PubMed).

Gheybi, Farrokhi, Ravanbod, Ostovar, Mehrzad, Nematollahi: "The correlation of CD19 + CD24 + CD38 + B cells and other clinicopathological variables with the proportion of circulating Tregs in breast cancer patients." in: Breast cancer (Tokyo, Japan), (2017) (PubMed).

Wang, Wang, Wang, Hong, Han, Chen, Chen: "HMGB1 induces lung fibroblast to myofibroblast differentiation through NF‑κB‑mediated TGF‑β1 release." in: Molecular medicine reports, Vol. 15, Issue 5, pp. 3062-3068, (2017) (PubMed).

Xuanfei, Hao, Zhujun, Yanming, Jianping: "Imidazoline I2 receptor inhibitor idazoxan regulates the progression of hepatic fibrosis via Akt-Nrf2-Smad2/3 signaling pathway." in: Oncotarget, Vol. 8, Issue 13, pp. 21015-21030, (2017) (PubMed).

Bu, Yu, Wu, Zhong, Yang, Chen: "Normalization of regulatory T cells, serum TGF-β1, and LTN after 5-aminolevulinic acid-photodynamic therapy in patients with condyloma acuminate." in: Experimental and therapeutic medicine, Vol. 13, Issue 6, pp. 3327-3332, (2017) (PubMed).

Cheng, Ge, Lan, Ma, Chi, Kuang, Sun, Zhao, Liu, Feng, Huang, Luo, Li, Zhang, Hu, Xu, Liu, Huo, Deng, Yang, Xi, Zhang, Siegenthaler, Chen: "Zoledronate dysregulates fatty acid metabolism in renal tubular epithelial cells to induce nephrotoxicity." in: Archives of toxicology, Vol. 92, Issue 1, pp. 469-485, (2017) (PubMed).

Wu, Yu, Cai, Lu, Jiang, Liu, Li, Feng, Gao, Zhu, Yu, Yuan, Cui: "Effects of increased Kindlin-2 expression in bladder cancer stromal fibroblasts." in: Oncotarget, Vol. 8, Issue 31, pp. 50692-50703, (2017) (PubMed).

Santagata, Napolitano, DAlterio, Desicato, Maro, Marinelli, Fragale, Buoncervello, Persico, Gabriele, Novellino, Longo, Pignata, Perdonà, Scala: "Targeting CXCR4 reverts the suppressive activity of T-regulatory cells in renal cancer." in: Oncotarget, Vol. 8, Issue 44, pp. 77110-77120, (2017) (PubMed).

Metzler, Pritz, Riml, Romani, Tuertscher, Steinbichler, Dejaco, Riechelmann, Dudás: "Separation of cell survival, growth, migration, and mesenchymal transdifferentiation effects of fibroblast secretome on tumor cells of head and neck squamous cell carcinoma." in: Tumour biology, Vol. 39, Issue 11, pp. 1010428317705510, (2017) (PubMed).

Zhuang, Lu, Shen, Huang, Shen, Zheng, Huang, Yan, Guo: "TGFβ1 secreted by cancer-associated fibroblasts induces epithelial-mesenchymal transition of bladder cancer cells through lncRNA-ZEB2NAT." in: Scientific reports, Vol. 5, pp. 11924, (2016) (PubMed).

Yılmazlar, Karabayırlı, Gözdemir, Usta, Peker, Namuslu, Erdamar: "The effect of positive end-expiratory pressure on inflammatory cytokines during laparoscopic cholecystectomy." in: Saudi medical journal, Vol. 36, Issue 11, pp. 1358-62, (2016) (PubMed).

Jiang, Chen, Wang, Lin, Hu, Fang: "Changes in interleukin-17 and transforming growth factor beta 1 levels in serum and bronchoalveolar lavage fluid and their clinical significance among children with asthma." in: Translational pediatrics, Vol. 2, Issue 4, pp. 154-9, (2016) (PubMed).

Zhang, Chen, Chen, Meng, Xiong, Liu, Jin, Tao, Wu, Sun: "TGF-β/BAMBI pathway dysfunction contributes to peripheral Th17/Treg imbalance in chronic obstructive pulmonary disease." in: Scientific reports, Vol. 6, pp. 31911, (2016) (PubMed).

Hu, Xu, Yao, Hong, Wu, Pei, Chang, Ding, Gao, Ye, Wang, Hei, Zhou: "MiR-663 inhibits radiation-induced bystander effects by targeting TGFB1 in a feedback mode." in: RNA biology, Vol. 11, Issue 9, pp. 1189-98, (2015) (PubMed).

Li, Pan, Qiu: "Imbalances of Th17 and Treg cells and their respective cytokines in COPD patients by disease stage." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 5324-9, (2015) (PubMed).

Wang, Yao, Xiong, Wu, Tang, Li: "Evaluation of the inflammatory response in macrophages stimulated with exosomes secreted by Mycobacterium avium-infected macrophages." in: BioMed research international, Vol. 2015, pp. 658421, (2015) (PubMed).

Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." in: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1

Background:

Background: Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative ion of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.

Gene Full Name: transforming growth factor beta 1

Gene ID: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints