TGFB1 ELISA Kit

Catalog No. ABIN6960086

Product Details TGFB1 ELISA Kit

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TGFb1 in rat serum, platelet-poor plasma, tissue homogenates, cell culture supernates.

Sample Type: Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1)

Sensitivity: 6.0 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Application Details

Comment:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Reagent Preparation:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 1,000pg/mL. Prepare 7 tubes containing 0.5 mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Sample Preparation:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage: 4 °C/-20 °C

Storage Comment:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Expiry Date: 6 months

References for TGFB1 ELISA Kit (ABIN6960086)

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Li, Yao, Hou, Zhang, Bao, Chen, Wang, Yue, Li, Zhang, Hao: "Crystalline Silica Promotes Rat Fibrocyte Differentiation in Vitro, and Fibrocytes Participate in Silicosis in Vivo." in: Biomedical and environmental sciences : BES, Vol. 30, Issue 9, pp. 649-660, (2018) (PubMed).

Li, Xiu, Wang, Brigstock, Sun, Qu, Gao: "Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas After Chronic Alcohol Exposure." in: Alcoholism, clinical and experimental research, Vol. 42, Issue 2, pp. 306-314, (2018) (PubMed).

Dik, Baş, Yazıhan: "The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats." in: Canadian journal of physiology and pharmacology, Vol. 95, Issue 5, pp. 604-609, (2017) (PubMed).

Kabel, Al-Shehri, Al-Talhi, Abd Elmaaboud: "The promising effect of linagliptin and/or indole-3-carbinol on experimentally-induced polycystic ovarian syndrome." in: Chemico-biological interactions, Vol. 273, pp. 190-199, (2017) (PubMed).

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Ni, Shi, Li, Chen, Li, Li, Zhu, Zhu, Fan: "Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 7083016, (2017) (PubMed).

Wang, Li, Wang, Zhang, Zhao, Fu, Han, Zhou, Ge: "Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3197320, (2017) (PubMed).

Abd El Motteleb, Ibrahim, Elshazly: "Sildenafil protects against bile duct ligation induced hepatic fibrosis in rats: Potential role for silent information regulator 1 (SIRT1)." in: Toxicology and applied pharmacology, Vol. 335, pp. 64-71, (2017) (PubMed).

Kabel, Elkhoely: "Ameliorative potential of fluoxetine/raloxifene combination on experimentally induced breast cancer." in: Tissue & cell, Vol. 48, Issue 2, pp. 89-95, (2016) (PubMed).

Zhao, Zhang, Chai, Li, Cui, Wang, Meng, Liu, Wang, Li, Bai, Xiao: "Oxymatrine attenuates CCl4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathways." in: International immunopharmacology, Vol. 36, pp. 249-55, (2016) (PubMed).

Wang, Lu, Zhang, Wang, Liu, Zhang, Geng, Shan: "Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 40, Issue 3-4, pp. 465-476, (2016) (PubMed).

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Maheshwari, Balaraman, Sen, Shukla, Seth: "Effect of concomitant administration of coenzyme Q10 with sitagliptin on experimentally induced diabetic nephropathy in rats." in: Renal failure, Vol. 39, Issue 1, pp. 130-139, (2016) (PubMed).

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Huang, Wang, Guo, Li, Yan, Yu, Wei, Li: "Effect of combined treatment with rosuvastatin and protein kinase C?2 inhibitor on angiogenesis following myocardial infarction in diabetic rats." in: International journal of molecular medicine, Vol. 35, Issue 3, pp. 829-38, (2015) (PubMed).

Kabel, Abd Elmaaboud, Albarraq: "Ameliorative potential of omega 3 fatty acids and HMG-CoA reductase inhibitors on experimentally-induced non-alcoholic steatohepatitis." in: Prostaglandins, leukotrienes, and essential fatty acids, Vol. 96, pp. 1-9, (2015) (PubMed).

Lemini, Ruiz-Herrera, Ledesma-Colunga, Díaz-Lezama, De Los Ríos, López-Barrera, Méndez, Martínez de la Escalera, Macotela, Clapp: "Prolactin anterior pituitary expression and circulating levels are reduced in obese and diabetic rats: role of TGF-? and TNF-?." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 308, Issue 9, pp. R792-9, (2015) (PubMed).

Wang, Cao, Cui, Kong, Tian, Cai, Wu, Han, Zhao, Xia: "Salvia Miltiorrhiza Bge.f.alba Ameliorates the Progression of Monocrotaline-Induced Pulmonary Hypertension by Protecting Endothelial Injury in Rats." in: The Tohoku journal of experimental medicine, Vol. 236, Issue 2, pp. 155-62, (2015) (PubMed).

Marshall-Webb, Fosh: "Gas-containing gallstones: a radiological finding." in: ANZ journal of surgery, (2015) (PubMed).

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: Transforming Growth Factor Beta 1 (TGFb1) (TGFB1 Products)

Synonyms: CED ELISA Kit, DPD1 ELISA Kit, LAP ELISA Kit, TGFB ELISA Kit, TGFbeta ELISA Kit, TGF-beta ELISA Kit, TGF-BETA-1 ELISA Kit, TGF-beta5 ELISA Kit, ced ELISA Kit, dpd1 ELISA Kit, lap ELISA Kit, tgf-beta ELISA Kit, tgfb ELISA Kit, tgfb5 ELISA Kit, tgfbeta ELISA Kit, TGF-beta1 ELISA Kit, TGFbeta1 ELISA Kit, Tgfb ELISA Kit, Tgfb-1 ELISA Kit, ai39657 ELISA Kit, tgfb1 ELISA Kit, wu:fb13a07 ELISA Kit, xx:ai39657 ELISA Kit, TGFB1 ELISA Kit, csd ELISA Kit, cdb1 ELISA Kit, cdg2 ELISA Kit, csd1 ELISA Kit, csd2 ELISA Kit, csd3 ELISA Kit, ebmd ELISA Kit, lcd1 ELISA Kit, bigh3 ELISA Kit, cdgg1 ELISA Kit, betaig-h3 ELISA Kit, TGFB4 ELISA Kit, transforming growth factor beta 1 ELISA Kit, transforming growth factor beta-1 ELISA Kit, transforming growth factor beta 1 L homeolog ELISA Kit, transforming growth factor, beta 1 ELISA Kit, transforming growth factor, beta 1a ELISA Kit, transforming growth factor beta induced L homeolog ELISA Kit, TGFB1 ELISA Kit, Tgfb1 ELISA Kit, tgfb1.L ELISA Kit, tgfb1a ELISA Kit, tgfbi.L ELISA Kit

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints