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IFN alpha A ELISA Kit

Cow IFN alpha A ELISA Kit Colorimetric assay for quantification of Cow IFN alpha A in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951055

Quick Overview for IFN alpha A ELISA Kit (ABIN6951055)

Target

See all IFN alpha A (IFNAA) products
IFN alpha A (IFNAA) (Interferon alpha-A (IFNAA))

Reactivity

Cow

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
  • Purpose

    Bovine IFN-alpha A ELISA Kit.

    Analytical Method

    Quantitative

    Characteristics

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Components

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Material not included

    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Plate

    Pre-coated

    Protocol

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Restrictions

    For Research Use only
  • Expiry Date

    6 months
  • Target See all IFN alpha A (IFNAA) products

    IFN alpha A (IFNAA) (Interferon alpha-A (IFNAA))

    Alternative Name

    IFN-alpha A

    Gene ID

    515951

    UniProt

    P05007
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