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IFN alpha A ELISA Kit
Cow IFN alpha A ELISA Kit Colorimetric assay for quantification of Cow IFN alpha A in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Quick Overview for IFN alpha A ELISA Kit (ABIN6951055)
Target
See all IFN alpha A (IFNAA) products
IFN alpha A (IFNAA)
(Interferon alpha-A (IFNAA))
Reactivity
Cow
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details IFN alpha A ELISA Kit
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Purpose
Bovine IFN-alpha A ELISA Kit.
Analytical Method
Quantitative
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Expiry Date
6 months
Target Details for IFN alpha A
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IFN alpha A (IFNAA)
(Interferon alpha-A (IFNAA))
Alternative Name
IFN-alpha A
Gene ID
515951
UniProt
P05007
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