ACE2 ELISA Kit

Catalog No. ABIN6952471

This Human ACE2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human ACE2.

Quick Overview for ACE2 ELISA Kit (ABIN6952471)

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.025 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Cell Culture Cells, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Product Details ACE2 ELISA Kit

Minimum Detection Limit: 0.025 ng/mL

Purpose: Human ACE-2 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects Human ACE-2. Other species not determined.

Characteristics: Human ACE-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water/li>
• Precision pipettes to deliver 2 μL to 1 μL volumes/li>
• Adjustable 1-25 μL pipettes for reagent preparation/li>
• 100 μL and 1 liter graduated cylinders/li>
• Tubes to prepare standard and sample dilutions/li>
• Absorbent paper/li>
• Microplate reader capable of measuring absorbance at 450nm/li>
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Sample Preparation:

Recommended Dilution (serum & plasma):8 fold

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Target Details for ACE2

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE-2

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour