ACE2 ELISA Kit
Quick Overview for ACE2 ELISA Kit (ABIN6952503)
Target
See all ACE2 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- This assay employs a two-site sandwich ELISA to quantitate ACE2 in samples.
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Analytical Method
- Quantitative
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Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit
ACE2 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 Reactivity: Human Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 Reactivity: Human Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 Reactivity: Mouse Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 Reactivity: Mouse AA 18-740 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 Reactivity: Human Colorimetric Sandwich ELISA 0.025 ng/mL - 20 ng/mL Cell Culture Cells, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 200 μL
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Plate
- Pre-coated
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Protocol
- An antibody specific for ACE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Restrictions
- For Research Use only
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- ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
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Alternative Name
- ACE2
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Pathways
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
Target See all ACE2 ELISA Kits
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