HCP ELISA Kit

Catalog No. ABIN7538037

This Colorimetric ELISA kit is designed for the quantitative measurement of Chinese Hamster HCP.

Quick Overview for HCP ELISA Kit (ABIN7538037)

Target: HCP (Host Cell Proteins (HCP))

Reactivity: Chinese Hamster

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.125 ng/mL – 100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant

Product Details HCP ELISA Kit

Minimum Detection Limit: 3.125 ng/mL

Purpose: The kit is intended for the quantitative determination of host cell protein impurities in Cell culture supernatants or host cell protein (HCP) contamination in end-product expressed by Chinese Hamster Ovary (CHO) expression systems.

Analytical Method: Quantitative

Cross-Reactivity (Details): No cross-reaction exists between the homologous antigens.

Sensitivity: 0.2 ng/mL

Characteristics: This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.

Components: Pre-Coated Microplate (Detachable), Standard (Stock Solution), Detection antibody (100x), TMB Substrates, Stop Solution, Wash Solution (100x), Diluent Buffer (10x), Plate Sealer

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 µL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra Variation%: 3.1-4.5, Inter Variation%: 6.0-9.1

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for HCP

Target: HCP (Host Cell Proteins (HCP))

Alternative Name: Host Cell Proteins