Anti- Chlamydia Pneumoniae Antibody (Cpn-IgM) ELISA Kit

Catalog No. ABIN7666550

Human ELISA Kit Colorimetric assay for quantification of Human .

Quick Overview for Anti- Chlamydia Pneumoniae Antibody (Cpn-IgM) ELISA Kit (ABIN7666550)

Target: Anti- Chlamydia Pneumoniae Antibody (Cpn-IgM)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details

Purpose: anti-Chlamydia pneumoniae IgM - ELISA kit for the detection of IgM antibodies to Chlamydia pneumoniae in human serum and plasma.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips in the handling frame coated with the antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control serum, r.t.u. NC 1 vial
• 1.3 mL Positive control serum, r.t.u. PC 1 vial
• 2.0 mL Calibrator, r.t.u. 1) CAL 1 vial
• 13 mL Anti-human IgM antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ 1 vial
• 2 mL RF sorbent 2), 25x concentrated RF SORB 25x 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
• 13 mL Stop solution, r.t.u. STOP 1 vial
• Instruction manual
• Certificate
• of quality 1) r.t.u., ready to use 2) Goat anti-human IgG globulin

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-Chlamydia pneumoniae IgM is a solid-phase immunoanalytical test. The purified, homogeneous native antigen is fixed to each well of the microtiterstrips. Specific antibodies present in the patient's sample are bound during the first incubation step. After removing unbound material by washing, the presence of the specific antibodies is detected using anti-human IgM conjugate during the second incubation. The unbound peroxidase conjugate is then removed and TMB substrate is added, resulting in the development of a blue colour in positive samples. The enzyme reaction is terminated by addition of the stop solution (colour change to yellow). The intensity of the yellow colour thus developed is proportional to the concentration of antibodies in the sample. 2

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: The ELISA kit should be used within three months after opening. a. Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Under these conditions, the expiration period of the entire kit is indicated on the central label, the expiration date of the individual components is indicated on their package labels. b. Store unused strips in the sealable pouch and keep the desiccant inside. c. Kits are shipped in cooling bags, the transport time up to 72 hours have no influence on expiration. If you find damage at any part of the kit, please inform the manufacturer immediately. d. Store undiluted sera in aliquots at -18 to -28 °C. Frequent freezing and thawing is not recommended. Undiluted serum samples could be stored at +2 to +10°C up to one week. e. Do not store diluted samples. Always prepare fresh. 14. USED SYMBOLS: number of tests Conformité Européenne - product meets the requirements of European legislation Conformité Européenne with number of notified person 1023 in vitro diagnostics σ standard deviation CV coefficient of variation OD optical density manufacturer expiration Lot of kit 10°C 2°C storage at +2°C to +10°C °C Celsius degree % percentage n number of tested samples A value of tested sample read usage instructions catalog number 10 15. FLOW CHART: Step 1 Prepare reagents and samples Step 2 Dispense 100 L/well controls and samples Incubate 30 minutes at 37 °C Wash 4 times (250 L/well), aspirate Step 3 Dispense 100 L/well of peroxidase conjugate Incubate 30 minutes at 37 °C Wash 4 times (250 L/well), aspirate Step 4 Dispense 100 L/well of chromogenic substrate (TMB) Incubate 15 minutes in dark at room temperature Step 5 Dispense 100 L/well of stop solution Step 6 Read the absorbance at 450 / 620-690 nm within 20 minutes General references: Roubalová K., Hrubá D., Janechková L.: Možnosti diagnostiky infekcí vyvolaných Chlamydia pneumoniae. Naše zkušenosti s diagnostikou Chlamydia pneumoniae u chronicky nemocných pacientů., Antibiotiká a rezistencia 2, 5, 2006, 13-17. P. Hejnar, D. Koukalová: Serodiagnostics of Chlamydial Infections-Significance of Positivity in IgA and/or IgM Antibody Classes only., Biomed. Papers 146(2), 33-35 (2002). Diagnostik von Chlamydien-Infektionen, J. Lab Med 2004, 28 (2): 144-153. Kazár, J.: Chlamýdiové pneumónie, Antibiotiká a rezistencia 2, 5, 2006, 9-12. 11 CHANGES 07.09.2023 Chapter 4 Increasing the volume of calibrator to 2 ml Date of the revision of manual: 07. 09. 2023 12

Target Details

Target: Anti- Chlamydia Pneumoniae Antibody (Cpn-IgM)

Alternative Name: Chlamydia pneumoniae IgM

Target Type: Antibody