VZV IgM ELISA Kit

Catalog No. ABIN7666553

Human VZV IgM ELISA Kit Colorimetric assay for quantification of Human VZV IgM.

Quick Overview for VZV IgM ELISA Kit (ABIN7666553)

Target: VZV IgM (Anti-Varicella Zoster Virus IgM (VZV IgM))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details VZV IgM ELISA Kit

Purpose: anti-VZV IgM - ELISA kit for detection IgM antibodies to varicella zoster virus (VZV) in human serum (plasma).

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips coated with specific antigen STRIPS Ag 1 microplate
• 1.3 mL Calibrator, r.t.u. 1) CAL 1 vial
• 1.3 mL Negative control serum, r.t.u. NC 1 vial
• 1.3 mL Positive control serum, r.t.u. PC 1 vial
• 2 mL RF sorbent2), 25x concentrated RF SORB 25x 1 vial
• 13 mL Anti-human IgM antibodies labelled with horseradish peroxidase r.t.u. (Px-conjugate) CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate (TMB substrate), r.t.u. TMB 1 vial
• 13 mL Stop solution, r.t.u. STOP 1 vial
• Instruction manual
• Quality control certificate 1) ready to use 2) Goat anti-human IgG globulin

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-VZV IgM assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a native antigen. Anti-VZV antibodies from serum samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away, those that formed complexes with the antigens are later on recognised by animal anti-human IgM antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for VZV IgM

Target: VZV IgM (Anti-Varicella Zoster Virus IgM (VZV IgM))

Alternative Name: VZV IgM

Target Type: Antibody