CMV IgM ELISA Kit

Catalog No. ABIN7666564

This Human CMV IgM ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CMV IgM.

Quick Overview for CMV IgM ELISA Kit (ABIN7666564)

Target: CMV IgM (Anti-Cytomegalovirus IgM Antibody (CMV IgM))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details CMV IgM ELISA Kit

Purpose: anti-CMV IgM - ELISA kit for the detection of IgM antibodies to human cytomegalovirus (CMV) in serum.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA 8-well break-away strips coated with a mixture of specific recombinant antigens STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control serum, r.t.u. PC 1 vial
• 2.0 mL Calibrator ,r.t.u. CAL 1 vial
• 13 mL Anti-human IgM antibodies labelled with horseraddish peroxidase r.t.u. CONJ-V 1 vial
• 55 mL Wash buffer 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
• 13 mL Stop solution r.t.u. STOP 1 vial
• 2 mL RF sorbent 2), 25x concentrated RF SORB 25x 1 vial
• Instruction manual
• Quality control certificate 1) (ready to use) 2) Goat anti-human IgG globulin /

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-CMV IgM assay is a solid-phase immunoanalytical test. The strips are coated with the mixture of recombinant antigens. Anti-CMV antibodies in serum samples bind to the mixture of immobilized antigens. Bound antibodies are later on recognised by animal anti-human IgM antibodies labelled with horseradish peroxidase. The amount of bound labeled antibodies is determined by color enzymatic reaction. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: The ELISA kit should be used within three months after opening. a. Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Under these conditions, the expiration period of the entire kit is indicated on the central label, the expiration date of the individual components is indicated on their package labels. b. Store unused strips in the sealable pouch and keep the desiccant inside. c. Kits are shipped in cooling bags, the transport time up to 72 hours have no influence on expiration. If you find damage at any part of the kit, please inform the manufacturer immediately. d. Store undiluted sera in aliquots at -18 to -28 °C. Frequent freezing and thawing is not recommended. Undiluted serum samples could be stored at +2 to +10°C up to one week. e. Do not store diluted samples. Always prepare fresh. 8

Target Details for CMV IgM

Target: CMV IgM (Anti-Cytomegalovirus IgM Antibody (CMV IgM))

Alternative Name: CMV IgM

Target Type: Antibody