HCP ELISA Kit

Catalog No. ABIN7764584

This Colorimetric ELISA kit is designed for the quantitative measurement of CHO cells HCP.

Quick Overview for HCP ELISA Kit (ABIN7764584)

Target: HCP (Host Cell Proteins (HCP))

Reactivity: CHO cells

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Biological Therapeutics, Cell Culture Supernatant, Purified Protein

Product Details HCP ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Purpose: ELISA Kit for the quantitative Measurement of host cell protein (HCP) residues in biopharmaceuticals by Chinese Hamster Ovary (CHO) expression systems

Analytical Method: Quantitative

Cross-Reactivity (Details): No cross-reaction exists between the homologous antigens.

Characteristics: CHO Host Cell Protein (CHO HCP) ELISA kit, G3

Components:

• 1. Pre-Coated Microplate (Detachable)
• 2. Standard (Stock Solution) -500µg/ml
• 3. Detection antibody (100x)
• 4. TMB Substrates
• 5. Stop Solution
• 6. Wash Solution (100x)
• 7. Diluent Buffer (10x)
• 8. Plate Sealer
• 9. Instruction Manual

Material not included:

• 10-1000μL pipettor
• Multichannel pipettor
• 1L sterilized deionized water or ultrarpure water
• Sterilized EP tubes
• Absorbent Paper
• Microplate reader
• High-speed centrifuge
• Mini Centrifuge
• Microplate washer or washing bottles
• Data analysis and graphing software

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by in putting the ODvalues of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.

Assay Precision: Intra-assay precision: 5-6 %
Inter-assay precision: 7-7.7 %

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for HCP

Target: HCP (Host Cell Proteins (HCP))

Alternative Name: Host Cell Proteins