Nipah Virus Glycoprotein G / Ephrin B2 Ligand Binding Assay Kit

Catalog No. ABIN7824747

Quick Overview for Nipah Virus Glycoprotein G / Ephrin B2 Ligand Binding Assay Kit (ABIN7824747)

Target: Ephrin B2 (EFNB2)

Reactivity: Human

Detection Method: Colorimetric

Application: Ligand Binding Assay (LBA)

Sample Type: Antibody, Plasma, Purified Protein, Recombinant Protein, Serum, Small Molecules

Analytical Method: Semi-Quantitative

Product Details

Purpose: The Nipah Virus Glycoprotein G / Ephrin B2 Ligand Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between Glycoprotein G and Ephrin B2 in the presence of potential inhibitors within 4 hours

Components:

• Pre-coated 96-well Microplate
• Wash Buffer
• Ligand Protein
• Detection Antibody
• HRP-Conjugated Anti-IgG
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Plate: Pre-coated

Protocol: Prepare all reagents and test reagent samples as instructed.
Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: The kit may be stored at 4°C up to 1 month from the date of shipment. Shelf life can be extended up to 6 months by storing protein and antibodies, at -20°C immediately upon delivery. All other kit components should be stored at 4°C.

Expiry Date: 6 months

Target Details

Target: Ephrin B2 (EFNB2)

Alternative Name: Ephrin B2

Gene ID: 1948

UniProt: Q4VCP5