CD74 ELISA Kit

Catalog No. ABIN7826590

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CD74.

Quick Overview for CD74 ELISA Kit (ABIN7826590)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.63 ng/mL - 40 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details CD74 ELISA Kit

Minimum Detection Limit: 0.63 ng/mL

Purpose: This ELISA kit applies to the in vitro quantitative determination of Human CD74 concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Cross-Reactivity (Details): This kit recognizes Human CD74 in samples. No significant cross-reactivity or interference between Human CD74 and analogues was observed

Characteristics: Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Components:

• Capture Antibody
• Reference Standard
• Micro ELISA pre-Plate
• Biotinylated Detection Antibody
• HRP Conjugate
• Manual

Material not included:

• Ancillary Reagents (ABIN7670776)
• Microplate reader with 450 nm wavelength filter
• High-precision transfer pipette
• EP tubes
• disposable pipette tips
• Incubator capable of maintaining 37 °C
• Deionized or distilled water
• Absorbent paper
• Loading slot

Application Details

Sample Volume: 100 µL

Assay Time: 1 d

Plate: Uncoated

Protocol:

1. Take out the Micro ELISA pre-Plate, add 100 µL Capture antibody working solution to each well. Incubate overnight at 2-8°C.
   Discard the liquid, add 200 µL ELISA Plate Blocking Buffer to each well. Incubate for 60 min at 37°C
2. Discard the liquid, add 100 µL standard or sample to the wells. Incubate for 90 min at 37°C
3. Discard the liquid, immediately add 100 µL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
4. Aspirate and wash the plate 3 times
5. Add 100 µL HRP conjugate working solution. Incubate foe 30 min at 37°C. Aspirate and wash the plate for 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37°C
7. Add 50 µL Stop Solution
8. Read the plate at 450 nm immediately. Calculation of the results

Assay Precision: Both intra-CV and inter-CV are < 10%

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Store all unopend components at -20°C for up to 12 months.

Expiry Date: 12 months

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74

Background: CD74, DHLAG, HLADG, II, Ia-GAMMA, CD74 Molecule

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints