beta 2 Adrenergic Receptor ELISA Kit

Catalog No. ABIN7830402

The Human beta 2 Adrenergic Receptor ELISA Kit (ABIN7830402) is a Colorimetric ELISA Kit designed to quantify Human beta 2 Adrenergic Receptor.

Quick Overview for beta 2 Adrenergic Receptor ELISA Kit (ABIN7830402)

Target: beta 2 Adrenergic Receptor (ADRB2) (Adrenergic, beta-2-, Receptor, Surface (ADRB2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details beta 2 Adrenergic Receptor ELISA Kit

Purpose: ADRB2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ADRB2 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for beta 2 Adrenergic Receptor

Target: beta 2 Adrenergic Receptor (ADRB2) (Adrenergic, beta-2-, Receptor, Surface (ADRB2))

Alternative Name: ADRB2

Background:

Synonyms: ADRB2R|ADRBR|B2AR|BAR|BETA2AR

Gene Description: adrenergic, beta-2-, receptor, surface

Gene ID: 154

UniProt: P07550

Pathways: cAMP Metabolic Process, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Brown Fat Cell Differentiation