Aryl Hydrocarbon Receptor ELISA Kit

Catalog No. ABIN7830403

Human Aryl Hydrocarbon Receptor ELISA Kit, Colorimetric assay for quantification of Human Aryl Hydrocarbon Receptor.

Quick Overview for Aryl Hydrocarbon Receptor ELISA Kit (ABIN7830403)

Target: Aryl Hydrocarbon Receptor (AHR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Aryl Hydrocarbon Receptor ELISA Kit

Purpose: AHR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AHR expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Aryl Hydrocarbon Receptor

Target: Aryl Hydrocarbon Receptor (AHR)

Alternative Name: AHR

Background:

Synonyms: bHLHe76

Gene Description: aryl hydrocarbon receptor

Gene ID: 196

UniProt: P35869

Pathways: Regulation of Cell Size