Androgen Receptor ELISA Kit

Catalog No. ABIN7830414

The Human Androgen Receptor ELISA Kit (ABIN7830414) is a Colorimetric ELISA Kit designed to quantify Human Androgen Receptor.

Quick Overview for Androgen Receptor ELISA Kit (ABIN7830414)

Target: Androgen Receptor (AR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Androgen Receptor ELISA Kit

Purpose: AR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AR expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: AR

Background:

Synonyms: AIS|DHTR|HUMARA|KD|NR3C4|SBMA|SMAX1|TFM

Gene Description: androgen receptor

Gene ID: 367

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding