ATP1A1 ELISA Kit

Catalog No. ABIN7830429

The Human ATP1A1 ELISA Kit (ABIN7830429) is a Colorimetric ELISA Kit designed to quantify Human ATP1A1.

Quick Overview for ATP1A1 ELISA Kit (ABIN7830429)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details ATP1A1 ELISA Kit

Purpose: ATP1A1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ATP1A1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: ATP1A1

Background:

Synonyms: MGC3285|MGC51750

Gene Description: ATPase, Na+/K+ transporting, alpha 1 polypeptide

Gene ID: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process