BCR ELISA Kit

Catalog No. ABIN7830442

Human BCR ELISA Kit, Colorimetric assay for quantification of Human BCR.

Quick Overview for BCR ELISA Kit (ABIN7830442)

Target: BCR (Breakpoint Cluster Region (BCR))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details BCR ELISA Kit

Purpose: BCR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BCR expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for BCR

Target: BCR (Breakpoint Cluster Region (BCR))

Alternative Name: BCR

Background:

Synonyms: ALL|BCR-ABL1|BCR1|CML|D22S11|D22S662|FLJ16453|PHL

Gene Description: breakpoint cluster region

Gene ID: 613

UniProt: P11274

Pathways: Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling