BID ELISA Kit

Catalog No. ABIN7830443

This Colorimetric ELISA kit is designed for the quantitative measurement of Human BID.

Quick Overview for BID ELISA Kit (ABIN7830443)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details BID ELISA Kit

Purpose: BID (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BID expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background:

Synonyms: FP497|MGC15319|MGC42355

Gene Description: BH3 interacting domain death agonist

Gene ID: 637

UniProt: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity