CD22 ELISA Kit
Quick Overview for CD22 ELISA Kit (ABIN7830446)
Target
See all CD22 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- CD22 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CD22 expression in cultured cells.
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Analytical Method
- Qualitative
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CD22 Molecule (CD22) ELISA Kit
CD22 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD22 Molecule (CD22) ELISA KitCD22 Reactivity: Human Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
CD22 Molecule (CD22) ELISA KitCD22 Reactivity: Mouse AA 22-702 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- CD22 (CD22 Molecule (CD22))
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Alternative Name
- CD22
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Background
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Synonyms: FLJ22814|MGC130020|SIGLEC-2|SIGLEC2
Gene Description: CD22 Molecule
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Gene ID
- 933
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UniProt
- P20273
Target See all CD22 ELISA Kits
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