B-Cell Linker ELISA Kit

Catalog No. ABIN7830447

The Human B-Cell Linker ELISA Kit (ABIN7830447) is a Colorimetric ELISA Kit designed to quantify Human B-Cell Linker.

Quick Overview for B-Cell Linker ELISA Kit (ABIN7830447)

Target: B-Cell Linker (BLNK)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details B-Cell Linker ELISA Kit

Purpose: BLNK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BLNK expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background:

Synonyms: BASH|BLNK-S|LY57|MGC111051|SLP-65|SLP65

Gene Description: B-cell linker

Gene ID: 29760

UniProt: Q8WV28

Pathways: BCR Signaling