CEBPB ELISA Kit

Catalog No. ABIN7830453

The Human CEBPB ELISA Kit (ABIN7830453) is a Colorimetric ELISA Kit designed to quantify Human CEBPB.

Quick Overview for CEBPB ELISA Kit (ABIN7830453)

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CEBPB ELISA Kit

Purpose: CEBPB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CEBPB expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CEBPB

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Alternative Name: CEBPB

Background:

Synonyms: C/EBP-beta|CRP2|IL6DBP|LAP|MGC32080|NF-IL6|TCF5

Gene Description: CCAAT/enhancer binding protein (C/EBP), beta

Gene ID: 1051

UniProt: P17676

Pathways: Interferon-gamma Pathway, Autophagy, Brown Fat Cell Differentiation