CAMK1 ELISA Kit

Catalog No. ABIN7830461

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CAMK1.

Quick Overview for CAMK1 ELISA Kit (ABIN7830461)

Target: CAMK1 (Calcium/calmodulin-Dependent Protein Kinase I (CAMK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CAMK1 ELISA Kit

Purpose: CAMK1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CAMK1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CAMK1

Target: CAMK1 (Calcium/calmodulin-Dependent Protein Kinase I (CAMK1))

Alternative Name: CAMK1

Background:

Synonyms: CAMKI|MGC120317|MGC120318

Gene Description: calcium/calmodulin-dependent protein kinase I

Gene ID: 8536

UniProt: Q14012

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Smooth Muscle Cell Migration