CARD6 ELISA Kit

Catalog No. ABIN7830465

The Human CARD6 ELISA Kit (ABIN7830465) is a Colorimetric ELISA Kit designed to quantify Human CARD6.

Quick Overview for CARD6 ELISA Kit (ABIN7830465)

Target: CARD6 (Caspase Recruitment Domain Family, Member 6 (CARD6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CARD6 ELISA Kit

Purpose: CARD6 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CARD6 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CARD6

Target: CARD6 (Caspase Recruitment Domain Family, Member 6 (CARD6))

Alternative Name: CARD6

Background:

Synonyms: CINCIN1

Gene Description: caspase recruitment domain family, member 6

Gene ID: 84674

UniProt: Q9BX69