Caspase 10 ELISA Kit

Catalog No. ABIN7830468

The Human Caspase 10 ELISA Kit (ABIN7830468) is a Colorimetric ELISA Kit designed to quantify Human Caspase 10.

Quick Overview for Caspase 10 ELISA Kit (ABIN7830468)

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Caspase 10 ELISA Kit

Purpose: CASP10 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CASP10 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Caspase 10

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Alternative Name: CASP10

Background:

Synonyms: ALPS2|FLICE2|MCH4

Gene Description: caspase 10, apoptosis-related cysteine peptidase

Gene ID: 843

UniProt: Q92851

Pathways: Apoptosis, Caspase Cascade in Apoptosis